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Technical Report No.31 『UHPLC applications in 0.1%Formic acid mobile phase』

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Although the analysis time can be significantly shortened by UHPLC, it takes a lot of time outside the analysis.

In particular, I think that adjusting the pH of the mobile phase is a daunting task.
Therefore, this report summarizes the applications that use 0.1% formic acid as the first method.

Since there is no troublesome work, you can prepare the next move by knowing the result early.
Alternatively, it is often the case that the objective can be achieved with 0.1% formic acid.

UHPLC and simple mobile phase preparation accelerate total speed.

 

Technical Rport No.31_E


Technical Report No.30

Biological substances

『Analysis of peptides/proteins and monoclonal antibodies by UHPLC』

 

This technical report focuses on the analysis of proteins and monoclonal antibodies. Moreover, it contains information from intact to fragmented peptides.

 

In addition to C1,  C8,  C18 were added to the lineup.

 

Here are the results that can only be obtained with 300Å.

 

Technical Rport No.30_E

 

 


No.112 Analysis of Polyphenols for HPLC

Foods

Column:

Develosil HSR AQ C18, 5um (4.6x150mm)

 

Application Data No.112


No.111 Analysis of Dicarboxylic acids for UHPLC

Other

Column:

Develosil UHPLC C30, 1.6um (2.0x50mm)

Develosil UHPLC C18, 1.6um (2.0x50mm)

Develosil UHPLC C8, 1.6um (2.0x50mm)

Application Data No.111


No.110 Analysis of compounds containing phosphate groups II

Biological substances

Column:

Develosil UHPLC C30, 1.6um (2.0x50mm)

Application Data No.110


No.109 Analysis of compounds containing phosphate groups I

Biological substances

Column:

Develosil UHPLC C30, 1.6um (2.0x50mm)

Application Data No.109


Technical Report No.24 「Analysis of Peptides with UHPLC」

Biological substances

We analyzed peptides using “Develosil UHPLC series” announced on Tuesday, October 23, 2018.

 

A major feature is that it is possible to change the analytical conditions that used ion-pairing reagents such as TFA until now to mild mobile phase such as ammonium formate.

Changing the pH from 3 to 7 makes it possible to correspond to LC / MS negative mode as well.

 

Technical Report No.24


No.108 Dicarboxylic acids

Other

Column:

Develosil HSR AQ C18, 5um (4.6x150mm)

Application Data No.108


No.107 Glutathione (Re, Ox)

Biological substances

Column:

Develosil HSR C18 Peptide, 5um (4.6x150mm)

Develosil HSR C18 Peptide, 3um (4.6x75mm)

Application Data No.107


No.106 Amyloid β-Protein

Biological substances

Amyloid β-protein is a peptide of about 40 amino acids, and it is said that accumulation of this substance is closely related to Alzheimer’s disease.

We analyzed amyloid β-protein using a Corona detector this time. In addition, this method can also be used for LC / MS by changing the column inner diameter to 2.0 mm.

Application Data No.106