Column:
Develosil HSR AQ C18, 5um (4.6x150mm)
Column:
Develosil HSR C18 Peptide, 5um (4.6x150mm)
Develosil HSR C18 Peptide, 3um (4.6x75mm)
Amyloid β-protein is a peptide of about 40 amino acids, and it is said that accumulation of this substance is closely related to Alzheimer’s disease.
We analyzed amyloid β-protein using a Corona detector this time. In addition, this method can also be used for LC / MS by changing the column inner diameter to 2.0 mm.
It is an analysis example in which insulin, BSA, monoclonal antibody, thyroglobulin are digested using a convenient kit.
In order to obtain more information, 2um particle size may be used, but in this data it is an analysis example using 3um particle size and a general-purpose HPLC system, so it is easy to analyze digests.
It is an analysis example of 20 amino acids derivatized by PTH (phenylthiohydatoin).
Combination of column + UHPLC system with particle size 3 um and length 250 mm can realize stable analysis and high accuracy analysis at all times without worrying about the pressure limit.
This is an example of analysis of oligodeoxythymidine using HPLC and UHPLC system.
Simply by selecting the column size and particle size according to your system you can easily analyze oligonucleic acids.
This data is not data transferred method from HPLC to UHPLC.
We analyzed 4 kinds of catechins and caffeine contained in tea.
The UHPLC condition transferred from HPLC method greatly reduces injection volume, solvent usage and analysis time.
In particular, a column with an inner diameter of 2.0 mm and a length of 50 mm reduces analysis time by 85% while maintaining sufficient separation to achieve high efficiency.
・Sample
dCTP, dTTP, dGTP, dATP
・Column
Develosil HSR AQ C18, 5um (4.6x150mm)
Develosil HSR AQ C18, 2um (2.0x50mm)
・Sample
Gly-Tyr, Val-Tyr-Val, Met-Enkephalin, Angiotensin II, Leu-
Enkephalin
・Column
Develosil HSR AQ C18, 5um (4.6x150mm)
Develosil HSR AQ C18,2um (2.0x50mm)
・Sample
Pyridoxine HCl, l-Phenylephrine, Acetaminophen, Guaifenesin, Diphenhydramine HCl, Ketoprofen, Flurbiprofen, Indomethacin, Ibuprofen
・Column
Develosil HSR AQ C18, 2um (2.0x50mm)