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Application Data No.113

Biological substances

Added application data No.114

「Analysis of intact proteins」


Application Data No.114_E

Technical Report No.30 『Analysis of peptides/proteins and monoclonal antibodies by UHPLC』

Biological substances

『Analysis of peptides/proteins and monoclonal antibodies by UHPLC』


This technical report focuses on the analysis of proteins and monoclonal antibodies. Moreover, it contains information from intact to fragmented peptides.


In addition to C1,  C8,  C18 were added to the lineup.


Here are the results that can only be obtained with 300Å.


Technical Rport No.30_E



No.110 Analysis of compounds containing phosphate groups II

Biological substances


Develosil UHPLC C30, 1.6um (2.0x50mm)

Application Data No.110

No.109 Analysis of compounds containing phosphate groups I

Biological substances


Develosil UHPLC C30, 1.6um (2.0x50mm)

Application Data No.109

Technical Report No.24 「Analysis of Peptides with UHPLC」

Biological substances

We analyzed peptides using “Develosil UHPLC series” announced on Tuesday, October 23, 2018.


A major feature is that it is possible to change the analytical conditions that used ion-pairing reagents such as TFA until now to mild mobile phase such as ammonium formate.

Changing the pH from 3 to 7 makes it possible to correspond to LC / MS negative mode as well.


Technical Report No.24

No.107 Glutathione (Re, Ox)

Biological substances


Develosil HSR C18 Peptide, 5um (4.6x150mm)

Develosil HSR C18 Peptide, 3um (4.6x75mm)

Application Data No.107

No.106 Amyloid β-Protein

Biological substances

Amyloid β-protein is a peptide of about 40 amino acids, and it is said that accumulation of this substance is closely related to Alzheimer’s disease.

We analyzed amyloid β-protein using a Corona detector this time. In addition, this method can also be used for LC / MS by changing the column inner diameter to 2.0 mm.

Application Data No.106

No.105 Digested Protein

Biological substances

It is an analysis example in which insulin, BSA, monoclonal antibody, thyroglobulin are digested using a convenient kit.

In order to obtain more information, 2um particle size may be used, but in this data it is an analysis example using 3um particle size and a general-purpose HPLC system, so it is easy to analyze digests.

Application Data No.105

No.103 Oligo Deoxy Thymidine

Biological substances

This is an example of analysis of oligodeoxythymidine using HPLC and UHPLC system.

Simply by selecting the column size and particle size according to your system you can easily analyze oligonucleic acids.

This data is not data transferred method from HPLC to UHPLC.

Application Data No.103

No.101 dNTP (4mixture) for UHPLC

Biological substances




Develosil HSR AQ C18, 5um (4.6x150mm)

Develosil HSR AQ C18, 2um (2.0x50mm)

Application Data No.101