Separation of sinigrin

Column: ZIC-pHILIC 100 x 4.6 mm Mobile phase: A) acetonitrile/5mM CH3COONH4 (90:10) B) 5mM CH3COONH4 Time 0min 60min %B 0% 40% Flow rate: 0.8mL/min Temperature: 30 C Detection: UV@215nm Sample: extraction of horseradish and sinigrin standard